anti fade mounting medium (Beijing Solarbio Science)
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Anti Fade Mounting Medium, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 99/100, based on 10827 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+fade+medium/Medium/pmc13105392-119-1-4
Average 99 stars, based on 10827 article reviews
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Incubation:Article Title: Effects of intermittent theta burst stimulation on the inflammatory response and cerebral blood flow in promoting neurovascular repair after ischemic stroke Article Snippet: The following primary antibodies were applied overnight at 4 °C: anti-NeuN (Abcam, 1:300, UK), anti-NeuN (Proteintech, 1:300, USA), anti-C3d (R&D Systems, 1:200, USA), anti-S100A10 (Proteintech, 1:200, USA), anti-GFAP (Proteintech, 1:300, USA), anti-GFAP (Cell Signaling Technology, 1:300), anti-CD31 (R&D Systems, 1:200, USA), anti-IBA1 (FUJIFILM WAKO, 1:300, Japan), anti-CD86 (Abcam, 1:200, UK), and anti-CD206 (R&D Systems, 1:300, USA) antibodies. .. After being washed three times with 1 × PBS (5 min each), the sections were incubated with an Alexa Fluor-conjugated secondary antibody (Abcam, 1:1000, UK) for 1.5 h. Finally, the sections were mounted with Article Title: Shuyu capsule improves estrous cycle-dependent depression-like behavior in premenstrual dysphoric disorder (PMDD) mice by increasing GABAergic neuronal activation and downregulating GABAAR δ subunit expression in the dorsal periaqueductal gray (dPAG) region Article Snippet: They were then washed and blocked using phosphate-buffered saline (PBS), which contains 5% (volume fraction) goat serum and 1% (vol‐ ume fraction) Triton X-100 (Solarbio, Beijing, China; T8200), at room temperature for 2 h. Then, slices were put into the primary and secondary antibody incuba‐ tion solutions. .. After incubation, slices were stuck to the glass slide and air-dried, evenly mounted with an Article Title: Unraveling the antidepressant mechanisms of JiaWeiSiNiSan: Insights from network pharmacology and experimental validation. Article Snippet: Ethnopharmacological relevance: Depression is a prevalent mental health disorder with significant global impact.. JiaWeiSiNiSan (JWSNS) has been employed for centuries in traditional Chinese medicine as a classical herbal formula for treating depression.. However, the precise mechanisms underlying its therapeutic effects remain Article Title: Adaptive immune dysregulation in depression: Cross-species evidence of CD4 + T cell dysfunction and pro-inflammatory pathway activation. Article Snippet: Subsequently, the tissue slices were incubated at room temperature in the dark for 2 h with FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L), a secondary antibody targeting rabbit IgG. .. Following the incubation period, the sections were mounted using an Staining:Article Title: Effects of intermittent theta burst stimulation on the inflammatory response and cerebral blood flow in promoting neurovascular repair after ischemic stroke Article Snippet: The following primary antibodies were applied overnight at 4 °C: anti-NeuN (Abcam, 1:300, UK), anti-NeuN (Proteintech, 1:300, USA), anti-C3d (R&D Systems, 1:200, USA), anti-S100A10 (Proteintech, 1:200, USA), anti-GFAP (Proteintech, 1:300, USA), anti-GFAP (Cell Signaling Technology, 1:300), anti-CD31 (R&D Systems, 1:200, USA), anti-IBA1 (FUJIFILM WAKO, 1:300, Japan), anti-CD86 (Abcam, 1:200, UK), and anti-CD206 (R&D Systems, 1:300, USA) antibodies. .. After being washed three times with 1 × PBS (5 min each), the sections were incubated with an Alexa Fluor-conjugated secondary antibody (Abcam, 1:1000, UK) for 1.5 h. Finally, the sections were mounted with Article Title: Bacterial vesicles from intratumoral L. salivarius enhance PD-1 blockade via FPR1-mediated macrophage polarization in gastric cancer. Article Snippet: After washing with PBS, the samples were incubated with fluorescence-conjugated secondary antibodies (Alexa Fluor 488 or 555; Cell Signaling Technology, USA) for 1 h at room temperature. .. Following additional PBS washes, nuclei were stained with DAPI and mounted using an Microscopy:Article Title: Shuyu capsule improves estrous cycle-dependent depression-like behavior in premenstrual dysphoric disorder (PMDD) mice by increasing GABAergic neuronal activation and downregulating GABAAR δ subunit expression in the dorsal periaqueductal gray (dPAG) region Article Snippet: They were then washed and blocked using phosphate-buffered saline (PBS), which contains 5% (volume fraction) goat serum and 1% (vol‐ ume fraction) Triton X-100 (Solarbio, Beijing, China; T8200), at room temperature for 2 h. Then, slices were put into the primary and secondary antibody incuba‐ tion solutions. .. After incubation, slices were stuck to the glass slide and air-dried, evenly mounted with an Fluorescence:Article Title: Unraveling the antidepressant mechanisms of JiaWeiSiNiSan: Insights from network pharmacology and experimental validation. Article Snippet: Ethnopharmacological relevance: Depression is a prevalent mental health disorder with significant global impact.. JiaWeiSiNiSan (JWSNS) has been employed for centuries in traditional Chinese medicine as a classical herbal formula for treating depression.. However, the precise mechanisms underlying its therapeutic effects remain Article Title: Adaptive immune dysregulation in depression: Cross-species evidence of CD4 + T cell dysfunction and pro-inflammatory pathway activation. Article Snippet: Subsequently, the tissue slices were incubated at room temperature in the dark for 2 h with FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L), a secondary antibody targeting rabbit IgG. .. Following the incubation period, the sections were mounted using an |
