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anti fade mounting medium  (Beijing Solarbio Science)


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    Structured Review

    Beijing Solarbio Science anti fade mounting medium
    Anti Fade Mounting Medium, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 99/100, based on 10827 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+fade+medium/Medium/pmc13105392-119-1-4
    Average 99 stars, based on 10827 article reviews
    anti fade mounting medium - by Bioz Stars, 2026-09
    99/100 stars

    Images

    Related Articles

    Incubation:

    Article Title: Effects of intermittent theta burst stimulation on the inflammatory response and cerebral blood flow in promoting neurovascular repair after ischemic stroke
    Article Snippet: The following primary antibodies were applied overnight at 4 °C: anti-NeuN (Abcam, 1:300, UK), anti-NeuN (Proteintech, 1:300, USA), anti-C3d (R&D Systems, 1:200, USA), anti-S100A10 (Proteintech, 1:200, USA), anti-GFAP (Proteintech, 1:300, USA), anti-GFAP (Cell Signaling Technology, 1:300), anti-CD31 (R&D Systems, 1:200, USA), anti-IBA1 (FUJIFILM WAKO, 1:300, Japan), anti-CD86 (Abcam, 1:200, UK), and anti-CD206 (R&D Systems, 1:300, USA) antibodies. .. After being washed three times with 1 × PBS (5 min each), the sections were incubated with an Alexa Fluor-conjugated secondary antibody (Abcam, 1:1000, UK) for 1.5 h. Finally, the sections were mounted with anti-fade medium containing DAPI (Solarbio, China) for nuclear staining. .. The samples were observed with an Olympus Fluorview-3000 confocal microscope (Olympus Optical Co., Japan), and ImageJ software (National Institutes of Health, USA) was used for quantitative analysis of the immunofluorescence intensity in each experimental group.

    Article Title: Shuyu capsule improves estrous cycle-dependent depression-like behavior in premenstrual dysphoric disorder (PMDD) mice by increasing GABAergic neuronal activation and downregulating GABAAR δ subunit expression in the dorsal periaqueductal gray (dPAG) region
    Article Snippet: They were then washed and blocked using phosphate-buffered saline (PBS), which contains 5% (volume fraction) goat serum and 1% (vol‐ ume fraction) Triton X-100 (Solarbio, Beijing, China; T8200), at room temperature for 2 h. Then, slices were put into the primary and secondary antibody incuba‐ tion solutions. .. After incubation, slices were stuck to the glass slide and air-dried, evenly mounted with an anti-fade medium with 4',6-diamidino-2-phenylindole dihydrochloride (DAPI) (Solarbio; S2110), and then immediately observed and imaged under a fluores‐ cent microscope. .. The primary and secondary antibody information is as follows: glutamate decarboxylase 67 (GAD67) (Millipore, Shanghai, China; MAB5406, 1∶200); cellular Finkel-Biskis-Jinkins murine osteo‐ sarcoma viral oncogene homolog (c-Fos) (Abcam, Shanghai, China; ab208942, 1:1000); goat anti-rabbit IgG H+L Alexa Fluor® 647 (Abcam; ab150083, 1∶500); and goat anti-mouse IgG H+L Alexa Fluor® 488 (Ab‐ cam; ab150113, 1∶1000).

    Article Title: Unraveling the antidepressant mechanisms of JiaWeiSiNiSan: Insights from network pharmacology and experimental validation.
    Article Snippet: Ethnopharmacological relevance: Depression is a prevalent mental health disorder with significant global impact.. JiaWeiSiNiSan (JWSNS) has been employed for centuries in traditional Chinese medicine as a classical herbal formula for treating depression.. However, the precise mechanisms underlying its therapeutic effects remain

    Article Title: Adaptive immune dysregulation in depression: Cross-species evidence of CD4 + T cell dysfunction and pro-inflammatory pathway activation.
    Article Snippet: Subsequently, the tissue slices were incubated at room temperature in the dark for 2 h with FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L), a secondary antibody targeting rabbit IgG. .. Following the incubation period, the sections were mounted using an anti-fade medium containing 4′,6-diamidino-2-phenylindole (S2110, Solarbio) to prevent fluorescence quenching. .. Subsequently, images were obtained using a fluorescence microscope (LEICA DMI3000B, LEICA DMi8 model, Wetzlar, Germany).

    Staining:

    Article Title: Effects of intermittent theta burst stimulation on the inflammatory response and cerebral blood flow in promoting neurovascular repair after ischemic stroke
    Article Snippet: The following primary antibodies were applied overnight at 4 °C: anti-NeuN (Abcam, 1:300, UK), anti-NeuN (Proteintech, 1:300, USA), anti-C3d (R&D Systems, 1:200, USA), anti-S100A10 (Proteintech, 1:200, USA), anti-GFAP (Proteintech, 1:300, USA), anti-GFAP (Cell Signaling Technology, 1:300), anti-CD31 (R&D Systems, 1:200, USA), anti-IBA1 (FUJIFILM WAKO, 1:300, Japan), anti-CD86 (Abcam, 1:200, UK), and anti-CD206 (R&D Systems, 1:300, USA) antibodies. .. After being washed three times with 1 × PBS (5 min each), the sections were incubated with an Alexa Fluor-conjugated secondary antibody (Abcam, 1:1000, UK) for 1.5 h. Finally, the sections were mounted with anti-fade medium containing DAPI (Solarbio, China) for nuclear staining. .. The samples were observed with an Olympus Fluorview-3000 confocal microscope (Olympus Optical Co., Japan), and ImageJ software (National Institutes of Health, USA) was used for quantitative analysis of the immunofluorescence intensity in each experimental group.

    Article Title: Bacterial vesicles from intratumoral L. salivarius enhance PD-1 blockade via FPR1-mediated macrophage polarization in gastric cancer.
    Article Snippet: After washing with PBS, the samples were incubated with fluorescence-conjugated secondary antibodies (Alexa Fluor 488 or 555; Cell Signaling Technology, USA) for 1 h at room temperature. .. Following additional PBS washes, nuclei were stained with DAPI and mounted using an anti-fade medium (Solarbio, China). .. Fluorescent and confocal images were obtained using a fluorescence microscope (Nikon Ti2-E) or a confocal laser scanning microscope (Nikon AXNIS-Elements 5.4).

    Microscopy:

    Article Title: Shuyu capsule improves estrous cycle-dependent depression-like behavior in premenstrual dysphoric disorder (PMDD) mice by increasing GABAergic neuronal activation and downregulating GABAAR δ subunit expression in the dorsal periaqueductal gray (dPAG) region
    Article Snippet: They were then washed and blocked using phosphate-buffered saline (PBS), which contains 5% (volume fraction) goat serum and 1% (vol‐ ume fraction) Triton X-100 (Solarbio, Beijing, China; T8200), at room temperature for 2 h. Then, slices were put into the primary and secondary antibody incuba‐ tion solutions. .. After incubation, slices were stuck to the glass slide and air-dried, evenly mounted with an anti-fade medium with 4',6-diamidino-2-phenylindole dihydrochloride (DAPI) (Solarbio; S2110), and then immediately observed and imaged under a fluores‐ cent microscope. .. The primary and secondary antibody information is as follows: glutamate decarboxylase 67 (GAD67) (Millipore, Shanghai, China; MAB5406, 1∶200); cellular Finkel-Biskis-Jinkins murine osteo‐ sarcoma viral oncogene homolog (c-Fos) (Abcam, Shanghai, China; ab208942, 1:1000); goat anti-rabbit IgG H+L Alexa Fluor® 647 (Abcam; ab150083, 1∶500); and goat anti-mouse IgG H+L Alexa Fluor® 488 (Ab‐ cam; ab150113, 1∶1000).

    Fluorescence:

    Article Title: Unraveling the antidepressant mechanisms of JiaWeiSiNiSan: Insights from network pharmacology and experimental validation.
    Article Snippet: Ethnopharmacological relevance: Depression is a prevalent mental health disorder with significant global impact.. JiaWeiSiNiSan (JWSNS) has been employed for centuries in traditional Chinese medicine as a classical herbal formula for treating depression.. However, the precise mechanisms underlying its therapeutic effects remain

    Article Title: Adaptive immune dysregulation in depression: Cross-species evidence of CD4 + T cell dysfunction and pro-inflammatory pathway activation.
    Article Snippet: Subsequently, the tissue slices were incubated at room temperature in the dark for 2 h with FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L), a secondary antibody targeting rabbit IgG. .. Following the incubation period, the sections were mounted using an anti-fade medium containing 4′,6-diamidino-2-phenylindole (S2110, Solarbio) to prevent fluorescence quenching. .. Subsequently, images were obtained using a fluorescence microscope (LEICA DMI3000B, LEICA DMi8 model, Wetzlar, Germany).



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    A CLSM images showing RB-Ag-GPs (red) internalized by PKH67-stained (green) Raw264.7, DC2.4, and Caco-2 cells with <t>DAPI-labeled</t> nuclei (blue) after incubation. B Ex vivo fluorescence imaging of BALB/c mice orally administered PBS or FITC-Ag-GPs, showing biodistribution in secondary lymphoid tissues (ILN, PP, MLN), IE, and major organs (heart, liver, spleen, lung, kidney, small intestine), with fluorescence intensity indicating Ag-GPs uptake. C – F Flow cytometry analysis of BMDCs activation. C Single-cell gating; D CD11c⁺ DC identification; E CD86⁺CD80⁺ double-positive cell selection; F Quantification of activated DCs after treatment with PBS, Ag, GPs, or Ag-GPs. Data are presented as mean ± SD ( n = 3), **** P < 0.0001.
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    Image Search Results


    A CLSM images showing RB-Ag-GPs (red) internalized by PKH67-stained (green) Raw264.7, DC2.4, and Caco-2 cells with DAPI-labeled nuclei (blue) after incubation. B Ex vivo fluorescence imaging of BALB/c mice orally administered PBS or FITC-Ag-GPs, showing biodistribution in secondary lymphoid tissues (ILN, PP, MLN), IE, and major organs (heart, liver, spleen, lung, kidney, small intestine), with fluorescence intensity indicating Ag-GPs uptake. C – F Flow cytometry analysis of BMDCs activation. C Single-cell gating; D CD11c⁺ DC identification; E CD86⁺CD80⁺ double-positive cell selection; F Quantification of activated DCs after treatment with PBS, Ag, GPs, or Ag-GPs. Data are presented as mean ± SD ( n = 3), **** P < 0.0001.

    Journal: NPJ Vaccines

    Article Title: Oral delivery of Trichinella spiralis antigens by yeast-derived β-glucan particles enhances anti-helminth immunity

    doi: 10.1038/s41541-026-01458-0

    Figure Lengend Snippet: A CLSM images showing RB-Ag-GPs (red) internalized by PKH67-stained (green) Raw264.7, DC2.4, and Caco-2 cells with DAPI-labeled nuclei (blue) after incubation. B Ex vivo fluorescence imaging of BALB/c mice orally administered PBS or FITC-Ag-GPs, showing biodistribution in secondary lymphoid tissues (ILN, PP, MLN), IE, and major organs (heart, liver, spleen, lung, kidney, small intestine), with fluorescence intensity indicating Ag-GPs uptake. C – F Flow cytometry analysis of BMDCs activation. C Single-cell gating; D CD11c⁺ DC identification; E CD86⁺CD80⁺ double-positive cell selection; F Quantification of activated DCs after treatment with PBS, Ag, GPs, or Ag-GPs. Data are presented as mean ± SD ( n = 3), **** P < 0.0001.

    Article Snippet: Raw264.7, DC2.4, and Caco-2 cells in 24-well plates were incubated with RB-Ag-GPs for 4 h, fixed with 4% paraformaldehyde, stained with PKH67 green fluorescent cell linker kit (PKH67GL, Sigma-Aldrich, Germany) for membrane labeling and DAPI anti-fade mounting medium (DAPI, HY-K1047, MedChemExpress, USA) for nuclear staining, followed visualized by CLSM.

    Techniques: Staining, Labeling, Incubation, Ex Vivo, Fluorescence, Imaging, Flow Cytometry, Activation Assay, Single Cell, Selection

    A CLSM images of RB-Ag-GPs (red) uptake in Raw264.7 and DC2.4 cells pre-treated with PBS or LA (10 μg/mL, 2 h). Nuclei counterstained with DAPI (blue). B Ex vivo fluorescence imaging of FITC-Ag-GPs distribution in LA or PBS-pretreated mice at 4 h post-administration. Tissues analyzed: ILN, PP, MLN, IE, and major organs. C Dectin-1 mRNA expression levels in intestinal tissues of immunized mice at 7 dpi. Data are presented as mean ± SD ( n = 3). ns not significant, *** P < 0.01, **** P < 0.0001. D , E Larvae per gram (LPG) of muscle burden and the reduction rate in mice following T. spiralis infection with vaccination at 35 dpi. Data are presented as mean ± SD ( n = 3), * P < 0.05, ** P < 0.01. F – H Ag-specific serum antibody levels (IgG, IgE, SIgA) in orally immunized mice. Data are presented as mean ± SD ( n = 3), * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001.

    Journal: NPJ Vaccines

    Article Title: Oral delivery of Trichinella spiralis antigens by yeast-derived β-glucan particles enhances anti-helminth immunity

    doi: 10.1038/s41541-026-01458-0

    Figure Lengend Snippet: A CLSM images of RB-Ag-GPs (red) uptake in Raw264.7 and DC2.4 cells pre-treated with PBS or LA (10 μg/mL, 2 h). Nuclei counterstained with DAPI (blue). B Ex vivo fluorescence imaging of FITC-Ag-GPs distribution in LA or PBS-pretreated mice at 4 h post-administration. Tissues analyzed: ILN, PP, MLN, IE, and major organs. C Dectin-1 mRNA expression levels in intestinal tissues of immunized mice at 7 dpi. Data are presented as mean ± SD ( n = 3). ns not significant, *** P < 0.01, **** P < 0.0001. D , E Larvae per gram (LPG) of muscle burden and the reduction rate in mice following T. spiralis infection with vaccination at 35 dpi. Data are presented as mean ± SD ( n = 3), * P < 0.05, ** P < 0.01. F – H Ag-specific serum antibody levels (IgG, IgE, SIgA) in orally immunized mice. Data are presented as mean ± SD ( n = 3), * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001.

    Article Snippet: Raw264.7, DC2.4, and Caco-2 cells in 24-well plates were incubated with RB-Ag-GPs for 4 h, fixed with 4% paraformaldehyde, stained with PKH67 green fluorescent cell linker kit (PKH67GL, Sigma-Aldrich, Germany) for membrane labeling and DAPI anti-fade mounting medium (DAPI, HY-K1047, MedChemExpress, USA) for nuclear staining, followed visualized by CLSM.

    Techniques: Ex Vivo, Fluorescence, Imaging, Expressing, Infection